INULINASE PURIFICATION PDF

Keywords: Characterization; Inulinase; Production; Purification; Streptomyces. 1. Introduction. Inulin is a polyfructan in plant consisting of linear chains. Aspergillus niger exo-inulinase purification by three phase partitioning. Inulinase (2, 1-β-D-fructan fructanohydrolase, EC ) hydrolyses inulin into. 42(4), August , pp. Purification and characterization of Inulinase from marine bacterium,. Bacillus cereus MU S Meenakshi1, S Umayaparvathi .

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Microbial inulinases formentation process, properties and applications. Idenlification of prokaryotic and eukaryotic signal peptides and predication of their cleavage site. Elution profile of the enzyme produced streptomyces grisenus after sephadex G filtration.

These results coincide with Allais et al. Data in Table 9 revealed the effect of metal ions on the activity of purified inulinase in the enzyme mixture. Effect of metal ions on the purified inulinase produced by Streptomyces grisenius. Therefore, microbial inulinases which can be induced by growing microorganisms, have a potential for industrial use in the production of fructose frminulin Edelman and Jefford, Hydrolysis of inulin from jerusalem artichake by inulinase immobilized on aminoethyl cellulose enzyme.

Biochemical, biophysical properties and structure-function analysis elucidates the substrate specificity of enzyme as well as its phylogenetic evolution and relationship.

The bacterial culture used in this study was the strongest inulinalytic strain among 50 isolates: Effect of incubation period on the inulinase activity of Streptomyces grisenus. Use of dinitrosalicylic acid reagent for determination of reducing sugar.

How to cite this article: Protein content was determined by the method of Lowry et al. Expression, purification and characterization of an exo-B-D-Fructosidase of Streptomyces mutans. Production of inulin fructotransferase depolymerizing by Artrobacter sp. Many micro organisms, including filamentous fungi, yeast, bacteria and streptomyces sp.

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N-Terminal amino acid sequencing: Effect of carbon source on the inulinase activity of Streptomyces grisenus. After separation, the proteins were transferred to a polyvinylidene difluoride membrane, and after staining with ponceau S solution, the inulinase protein band was cut from the membrane and subjected to amino acid sequence analysis.

Similar results were obtained YoKota et al. Inulinase can be found in plants and microorganisms. Results represented in Table 8 Indicated that the inulinase activity increased with increasing the substrate concentration until 1 mu and the activity was constant between 1- muinulin.

Production, Purification, Characterization and Applications of Fungal Inulinases | BenthamScience

Its production, properties and industrial applications. Pirification precipitate formed was obtained by centrifugation in an ultracentrifuge at The effect of carbon source on inulinase production was shown in Table 4. Assay of inulinase activity and protein content of each fraction were examined. Similar results were obtained by Burne et al.

Protein measurement with the folin phenol reagent. As shown in Table 1 the maximum activity was found on the 2nd day of incubation period. The production broth medium was optimized for maximum inulinase production by addition of 0.

The eluate was inklinase for protein content and inulinase activity. Close Print this page. Culture condition for inulinase production: Methods for characterization of Streptomyces species. Exocellular Inulinase of Bacillus subtilis Inulin was the best carbon source for inulinase production, while starch, maltose and lactose were inadequate. Y resistant to catabolite repression. Isolation and characlerization of bacterial strains with inulinase activity.

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Production, Purification, Characterization and Applications of Fungal Inulinases

Elsevier Applied Science, London, pp: Therefore, this study aimed to collate all information, particularly on fungal sources, characteristics, and applications of inulinases. The fermentation products from these groups of bacteria give rise to local and systemic health effects.

The optimum growth and inulinase activity occurred in the presence of 0. The activity units of the enzyme were determined from calibration curve of different concentrations of fructose solution purificatikn assay.

GNDuI produced high levels of extra cellular inlinase lnulinase 24 h at pH 7.

Purification, properties and comparison of invertase, exoinulinase and endoinulinase of Aspergillus ficuum. Effect of temperature on the activity of purificatikn inulinase from Streptomyces grisenus. Effect of temperature on the inulinase activity of Streptomyces grisenus.

Inulin, inulinases, purification, high fructose syrup, fructooligosaccharides, enzymes. Co-operative description of type culture of St. Co-operative description of type strain of Streptomyces. The results in Table 2 revealed that the maximum activity was observed at pH 7.

It was identified and confirmed according to description of ISP reported by Shirling and Gottliebaband and key of Bergey’s Manual of Williams et al.

The gel permeation chromatography on Sephadex G column provided large additional increase in the purity of the enzyme according to Ettalibi and Baratti