PDF | Gerbera (Gerbera jamesonii Bolus) is one of the most popular ornamental flowers worldwide and used both as cut flower and potted. PDF | Gerbera jamesonii (gerbera) is an important cut-flower in the global floricultural industry. Micropropagation is the main system used to. PDF | In present research, the effects of light quality on micropropagation of gerbera were investigated. The MS medium containing 1 mg L-1 BAP and mg .
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Studies of Gerbera clonal propagation could also be efficiently adapted for other ornamental plants. Takayama and Misawa reported that medium containing 0. Microprooagation were obtained from 8-week-old aseptic seedlings. Abstract Regeneration potentials in Gerbera jamesonii Bolus ex.
A potent cytokinin for woody plant tissue culture. Root formation occurred when explants were cultured on medium with higher auxin concentration and lower cytokinin concentration. Addition of auxins together with cytokinins becomes essential for shoot induction and multiplication depending on the microproopagation type.
Gerbera jamesonii is also used in the preparation of traditional Chinese medicine, tu-er-feng, for curing cold and also for treating rheumatism Ye et al.
They are planted outdoors in full sun and useful as cut flowers, pot plant and also microprkpagation plant. Callus is capable of forming adventitious roots. Structural, physiological and biochemical aspects.
Micropropagation of Gerbera (Gerbera jamesonii Bolus).
The regenerated plants gwrbera observed to be normal. Gerbera plantlets from in vitro cultivated capitulum explants. Tissues of explants generally show distinct planes of cell division, various specializations of cells and organization into specialized structures such as the vascular system.
The lowest shoot formation was observed when explants were cultured on MS medium supplemented with 0. Bigot reported that the addition of g L -1 activated charcoal in the culture medium showed vigorous rooting from excised shoots of Begoniax hiemalis.
De novo shoot organogenesis of pinus eldarica medw. In vitro propagated Gerbera plants were compared morphologically with the intact plant.
MICROPROPAGATION OF GERBERA
Factors affecting differentiation in vitro and mass propagation scheme for Begonia x hiemalis. The physiological status of the donor is determined by environmental conditions such as temperature, light intensity, day-length and light wavelength. BAP strongly enhanced regeneration of shoots in petiole explants of Gerbera jamesonii.
Springer-Verlag, New York, pp: Micropropagation selected somaclones Begonia Saintpaulia J. Different types of auxin and cytokinin combinations were used in order to obtain complete regeneration of Gerbera in vitro.
Through indirect organogenesis, multiple shoots can be produced in vitro from callus. Micropeopagation variations in micropropagated gerbera are frequently observed only with high concentrations of cytokinins in the culture medium but somaclonal variation is rare. Ornamental plants and woody plant species are also propagated commercially by axillary bud proliferation Mantell et al.
Adventitious shoots were obtained from petiole explants cultured on Murashige and Skoog MS medium supplemented with 2. Plantlets survival rate achieved was Micropropagation of ornamental plants-cut flowers.
The present study showed that only petiole explant produced optimum results for shoot formation. However, regeneration of root was not observed micropropagatiom explants were cultured on MS medium supplemented with BAP and 2, 4-D.
The first step towards de novo regeneration is to establish callus or cell suspension cultures. In vitro establishment and plantlet regeneration of Micropdopagation jamesonii Bolus ex. Many commercial ornamental plants are being propagated by in vitro culture on the culture medium containing auxins and cytokinins Preil, ; Rout and Jain, The main purpose for propagation of ornamental plants is for its aesthetic value.
The authors wish to thank the Institute of Biological Sciences and University of Malaya, Kuala Lumpur, Malaysia for providing all facilities and financial support.
Regenerated micropropxgation were rooted on Murashige and Skoog basal medium. Plant propagation by tissue culture technique is mainly aimed to produce plants with very high multiplication rates. Combination of auxin and cytokinin induces the formation of adventitious shoots and roots. However, in Gerbera jamesoniicytokinin alone in the medium induces the formation of microshoots.
Pakistan Journal of Biological Sciences Volume 11 The propagation rates via organogenesis can be much higher than axillary shoot proliferation Chun, The right combination of auxin and cytokinin in the culture medium determined the effectiveness of micro propagation of Gerbera shoots.
Numerous types of explants and protocols for micropropagation have been established and used for gerbera. Thus, improvements of plant quality need to be studied and more research needs to be done. Optimilization of Gerbera plantlet production from excised capitulum explants. However, higher addition of auxin compared to cytokinin in the culture medium resulted in the inhibition of shoot formation.