EcoRI restriction enzyme, 1. Restriction buffer, 1. Lambda DNA, uncut, 1. DNA size standard (HindIII lambda DNA digest), 1. Sample loading buffer, 5x, 1 ml, 1. Lambda DNA: Location of Sites. BglII. 0. . EcoRI. Nrul. o o. At ambient temperatures, in a solution containing purified Lambda-DNA these so- called ‘cos ends’ This is a BamHI-EcoRI restriction map of Lambda DNA.

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New Password Minimum of 8 characters Uppercase and lowercase letters At least one number. We offer numerous convenient solutions to meet your lab’s needs. Password Minimum of 8 characters Uppercase and lowercase letters At least one number. Communication error Reload Page. To read more click here. Students use their electrophoresis results to construct standard curves and determine the precise DNA fragment sizes generated by the different restriction enzymes.

Phage Lambda DNA EcoRI digest ready-to-use

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How can we help you? Each ecofi enzyme used in this kit cuts the lambda DNA several times, generating distinct sets of DNA restriction fragments of different sizes.

About Lambda DNA

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The lambda genome has approximately 48, base pairs. Learn more Functionality cookies These cookies remember choices you make such as your user name and language, or the region you are dago to improve your experience and provide enhanced, more personal features. In order to improve this site and our service to you, we currently use the WebTrends On Demand product and other similar products to track site usage.

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Phage Lambda DNA EcoRI digest ready-to-use – GENEON

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Since cos ends have complementary overhangs they are compatible for efficient ligation. Automated Nucleic Acid Purification. We use cookies to make our website easier for you to use. Kit contains sufficient materials for 8 student workstations 2—4 students per workstation. Reload Page If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the list price.

The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. A password reset email has been sent to the primary email address associated with your account.

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